BackgroundMetabolism alteration is a common complication of rheumatic arthritis (RA). This work investigated the reason behind RA-caused triglyceride (TG) changes.MethodsFresh RA patients'' whole blood was transfused into NOD-SCID mice. Metabolism-regulatory tissues were examined after sacrifice. To verify the findings, tissues of the rats with long-lasting adjuvant-induced arthritis (AIA) were analyzed. Some rats were injected with human plasma and GPIHBP1, and their blood TG was monitored. Various cells were stimulated by cytokines or rheumatic subjects'' serum. Some pre-adipocytes were cultured by human serum or in the presence of HUVEC cells and GPIHBP1.ResultsTG decrease occurred in blood and white adipose tissues (WAT) of the RA blood-transfused NOD-SCID mice and chronic AIA rats. Fatty acids (FA) oxidation in muscles was accelerated a bit, while TG catabolism status in their livers was varied. TNF-alpha, IL-1 beta, IL-6 and RA/AIA serum promoted expression of TG utilization-related enzymes and FA uptake transporters in pre-adipocytes, but barely affected LPL. Mild IL-6 stimulus promoted GPIHBP1 release of HUVEC cells. GPIHBP1 was increased in RA serum. This change can decrease blood TG in rats, which was overshadowed by an injection of excessive GPIHBP1. RA serum slightly inhibited LPL secretion in pre-adipocytes. Both HUVEC cells co-culture and GPIHBP1 supplement reduced LPL distribution on pre-adipocytes, and eliminated LPL activity difference between normal and RA serum-treated cells. No TG uptake difference was observed in these circumstances.ConclusionRA-associated inflammation induces GPIHBP1 secretion of endothelial cells, which facilitates blood TG hydrolysis and uptake to compensate the loss in WAT.
[1]Yijishan Hosp, Wannan Med Coll, Affiliated Hosp 1, Xinan Med Res Ctr, 2 West Zheshan Rd, Wuhu 241000, Anhui, Peoples R China.
[2]Wannan Med Coll, Affiliated Hosp 2, Dept Pharm, 10 Kangfu Rd, Wuhu 241000, Anhui, Peoples R China.
[3]Yijishan Hosp, Wannan Med Coll, Affiliated Hosp 1, Precis Med Ctr, Wuhu 241000, Peoples R China.
[4]Mohammed VI Polytech Univ, African Genome Ctr, Ben Guerir 43150, Morocco.
[5]Wannan Med Coll, Anhui Prov Key Lab Noncoding RNA Basic & Clin Tran, Wuhu 241000, Peoples R China.
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