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Long Noncoding RNA GCASPC, a Target of miR-17-3p, Negatively Regulates Pyruvate Carboxylase-Dependent Cell Proliferation in Gallbladder Cancer 期刊论文

编号：

a363f2a3-6b3e-4204-972e-a6b8c2351653
作者：

Ma, Mingzhe(马明哲)#^[1,2,3]Zhang, Yan(张妍)#^[4]Weng, Mingzhe(翁明哲)#^[1,2]Wang, Shouhua#^[1,2]Hu, Ye(胡靥)^[5]Hou, Zhaoyuan(侯照远)^[6]Qin, Yiyu^[1,2];Gong, Wei(龚伟)^[1,2]Zhang, YongJie(张永杰)^[7,8]Kong, Xiang(孔祥)*^[9]Wang, Jiandong(王健东)*^[1,2]Quan, Zhiwei(全志伟)*^[1,2]
语种：

英文
期刊：

CANCER RESEARCH ISSN：0008-5472 2016 年 76 卷 18 期 (5361 - 5371) ; SEP 15
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摘要：

Long noncoding RNAs (lncRNA) are being implicated in the development of many cancers. Here, we report the discovery of a critical role for the lncRNA GCASPC in determining the progression of gallbladder cancer. Differentially expressed lncRNAs and mRNAs between gallbladder cancer specimens and paired adjacent nontumor tissues from five patients were identified and validated by an expression microarray analysis. Quantitative real-time PCR was used to measure GCASPC levels in tissues from 42 gallbladder cancer patients, and levels of GCASPC were confirmed further in a separate cohort of 89 gallbladder cancer patients. GCASPC was overexpressed or silenced in several gallbladder cancer cell lines where molecular and biological analyses were performed. GCASPC levels were significantly lower in gallbladder cancer than adjacent nontumor tissues and were associated with tumor size, American Joint Committee on Cancer tumor stage, and patient outcomes. GCASPC overexpression suppressed cell proliferation in vitro and in vivo, whereas GCASPC silencing had opposite effects. By RNA pull-down and mass spectrometry, we identified pyruvate carboxylase as an RNAbinding protein that associated with GCASPC. Because GCASPC is a target of miR-17-3p, we confirmed that both miR-17-3p and GCASPC downregulated pyruvate carboxylase level and activity by limiting protein stability. Taken together, our results defined a novel mechanism of lncRNA-regulated cell proliferation in gallbladder cancer, illuminating a new basis for understanding its pathogenicity. (C) 2016 AACR.
推荐引用方式
GB/T 7714：

Ma Ming-zhe,Zhang Yan,Weng Ming-zhe, et al. Long Noncoding RNA GCASPC, a Target of miR-17-3p, Negatively Regulates Pyruvate Carboxylase-Dependent Cell Proliferation in Gallbladder Cancer [J].CANCER RESEARCH,2016,76(18):5361-5371.
APA：

Ma Ming-zhe,Zhang Yan,Weng Ming-zhe,Wang Shou-hua,&Quan Zhi-wei.(2016).Long Noncoding RNA GCASPC, a Target of miR-17-3p, Negatively Regulates Pyruvate Carboxylase-Dependent Cell Proliferation in Gallbladder Cancer .CANCER RESEARCH,76(18):5361-5371.
MLA：

Ma Ming-zhe, et al. "Long Noncoding RNA GCASPC, a Target of miR-17-3p, Negatively Regulates Pyruvate Carboxylase-Dependent Cell Proliferation in Gallbladder Cancer" .CANCER RESEARCH 76,18(2016):5361-5371.
入库时间：

9/2/2016 12:00:00 AM
更新时间：

9/2/2016 12:00:00 AM
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